Validating a Firefly Luciferase-Based High-Throughput Screening Assay for Antimalarial Drug Discovery
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چکیده
منابع مشابه
A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery
Objective(s): Here, a reporter cell line containing two reporter vectors were developed, in order to monitor the Human T-Lymphotropic Virus type1(HTLV-1) infectivity and the cell viability simultaneously. Materials and Methods: The reporter cell line was constructed by stably transfected baby hamster's kidney cell line (BHK-21), with the genomes expressing two different reporters in separate pl...
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Drug screening is a long and costly process confronted with low productivity and challenges in using animals, which limit the discovery of new drugs. To improve drug screening efficacy and minimize animal testing, recent efforts have been dedicated to developing cell-based high throughput screening (HTS) platforms that can provide more relevant in vivo biological information than biochemical as...
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In order to identify the most attractive starting points for drugs that can be used to prevent malaria, a diverse chemical space comprising tens of thousands to millions of small molecules may need to be examined. Achieving this throughput necessitates the development of efficient ultra-high-throughput screening methods. Here, we report the development and evaluation of a luciferase-based pheno...
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ژورنال
عنوان ژورنال: ASSAY and Drug Development Technologies
سال: 2012
ISSN: 1540-658X,1557-8127
DOI: 10.1089/adt.2011.0378